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KMID : 0379119940220040332
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Korean Journal of Mycology
1994 Volume.22 No. 4 p.332 ~ p.337
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Effect of Various Carbon Sources on the Development of Aspergillus nidulans with velA+ or velA1 allele
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이영훈/Lee, Young Hoon
채건상/한동민/한유정/장광엽/Chae, Keon Sang/Han, Dong Min/Han, Yoo Jeong/Jahng, Kwang Yeop
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Abstract
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Under standard condition (Ban, er al., 1990: glucose 1%-nitrate 0.1% minimal medium, 30 ㎖ in 9 ㎝ plate, 10^6 cells of inoculum per plate), wild type of Aspergillus nidulans developed both sexual and asexual organs in ballance, while velA1 mutant developed asexual ones preferentially. Increase of glucose concentration did not signiticantly affect the asexual sporulation. However, development of sexual organs were largely affected. It was greatly enhanced when favorable nitrogen source, for example, casein hydrolysate was added, which is contrary to the case of Neurospora or Saccharomyces where limitation of N source induces sexual development. On most of moderate C. sources asexual development in velA^+ strain was largely inhibited except acetate on which only asexual spores were produced, while that in velA1 mutant strain was not affected. Lactose promoted the sexual development even in velA1 mutant, indicating that lactose itself or its metabolic intermediate may induce sexual development independent of allelic state of velA gene. On other moderate favorable C sources, glycerol, galactose and ethanol, asexual development was largely inhibited in velA^+ strain but not in velA1 mutant strain. Sexual organs were, however, never produced on acetate. These results suggested that asexual development of wild type is largely dependent on C sources and the velA gene is involved in the repression of asexual development in not-enough-grown (non-competent) thalli resulting in preferential progression of sexual development.
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